{"id":264,"date":"2015-12-02T19:03:28","date_gmt":"2015-12-02T19:03:28","guid":{"rendered":"http:\/\/teclu.chem.uaic.ro\/brindusapetre\/?page_id=264"},"modified":"2016-12-30T10:18:56","modified_gmt":"2016-12-30T10:18:56","slug":"project-pn-ii-ru-te-2014-4-0920","status":"publish","type":"page","link":"http:\/\/teclu.chem.uaic.ro\/brindusapetre\/project-te\/project-pn-ii-ru-te-2014-4-0920\/","title":{"rendered":"Project PN-II-RU-TE-2014-4-0920"},"content":{"rendered":"

Project TE<\/h1>\n
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Project information<\/h1>\n

Source of funding :\u00a0<\/strong>National Council of Scientific Research in Higher Education \u2013 CNCSIS, UEFSCDI.<\/p>\n

Project Code: <\/strong>PN-II-RU-TE-2014-4-0920.<\/p>\n

Number of contract :<\/strong> 121 \/ 01\/10\/2015<\/p>\n

Project title :\u00a0<\/strong>The characterization of pathophysiological protein modifications by bio-affinity mass spectrometric methods<\/p>\n<\/div>\n

Project Description<\/h1>\n

Abstract<\/p>\n

All pathophysiological disorders from neurodegenerative diseases to diabetes, heart diseases, cancer and rare diseases are characterized by damage in structure or function of essential biological macromolecules (e.g. peptides, proteins and enzymes). The proposed research project comprises two major objectives: (i), Neurological Abeta peptide involved in Alzheimer\u2019s disease (AD) and the effect of oxidative stress will be investigated by structural mass spectrometry (MS) in combination with bioaffinity strategies. The major interest is to obtain detailed understanding of oxidative Abeta aggregation mechanism, investigating toxic oligomeric species formed in the initial step of this process. Synthetic nitrated Abeta peptides will be used for identification, characterization and quantification of oligomers in AD mouse models. (ii), Moreover we propose to start developing new mass spectrometric based approaches for early diagnostic of treatable rare diseases. Lysosomal storage diseses (LSD\u2019s) are a large group of rare metabolic disorders caused by the deficiency of single enzymes in metabolizing biopolymer substrates. Synthetic substrates for a few defected lysosomal enzymes will be synthesized and used for fluorimetric and mass spectrometric based diagnostic approaches. Together our data will improve the quality of methods and will increase further research interest regarding intrigue parallels existing between pathology of AD and lysosomal Niemann-Pick rare disorder.<\/p>\n

Team members<\/h2>\n

Project Manager<\/strong>: Lecturer Dr. Br\u00eendu\u0219a Alina Petre<\/p>\n

Other Members:\u00a0<\/strong><\/p>\n

C.S.I Dr. Lucian Hri\u021bcu<\/p>\n

C.S.II Dr. Cristina Dimitriu<\/p>\n

M.D. Dr. Valeriu Vasile Lupu (3 months)<\/p>\n

Drd. Laura Ion<\/p>\n

Drd. Claudia Andrie\u0219<\/p>\n

Objectives<\/h2>\n

The proposed project\u00a0has the following two major objectives:<\/p>\n

I. Characterization of oligomeric A\u00df species by bio-affinity mass spectrometry and the effect of their disintegration under the action of natural compounds.<\/p>\n

II. Development of strategies to diagnose lysosomal rare diseases by fluorimetry and mass spectrometry.<\/p>\n

 <\/p>\n

Dissemination<\/h2>\n

Publications in which the financial support of the project is acknowledged<\/em><\/strong><\/p>\n

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  1. Postu, J. Noumedem, O. Cioanca, M. Hancianu, M. Mihasan, B. A. Petre*, L. Hritcu*, (2016) Lactuca capensis ameliorate beta-amyloid induced memory impairment and oxidative stress in animal model of Alzheimer\u2019s disease, Journal of Cellular and Molecular Medicine<\/em> \u2013 under review <\/em>(IF 4.938)<\/strong><\/li>\n
  2. Murariu, M., Ion, L., Ciobanu, C.I., Petre, B.A. and Drochioiu, G. (2016) MS, CD, and FT-IR characterization of five newly synthesized histidine-containing Ala- and Gly-based peptides. Revue Roumaine de Chimie \u2013 under review (IF 0.250)<\/strong><\/em><\/li>\n
  3. Ion, L., Cozma, C., Andrie\u0219, C., Maeser, S., Przybylski, M., Petre, B.A. Diagnosis by MRM-MS of Maroteaux-Lamy Syndrome in Dried Blood Spots Using a New Substrate. Current Topics in Medicinal Chemistry<\/em> \u2013 in progress<\/em><\/strong> (IF 2.91<\/strong>)<\/li>\n
  4. Ioni\u021b\u0103, P. A. Postu, Laura I. M. Miha\u0219an, O. Cioanca, M. Hancianu, B. A. Petre*, L. Hritcu* Hypericumperforatum <\/em>aqueous extract enhance memory formation via decreasing oxidative damage in the rat hippocampus, Biomedicine&Pharmacotherapy – in progress <\/strong><\/em>( IF 2.326)<\/strong><\/li>\n
  5. Andrie\u015f, \u015e. Sl\u0103mnoiu, L. Ion, A. Neam\u0163u and B. A. Petre, (2016), Cross Reactivity of Anti Nitro-Tyrosine Antibodies to Oxidative Modified Tyrosine Containing Peptides by Using a SAW-Biosensor Assay, PlosOne<\/em> \u2013 in progress<\/em><\/strong><\/li>\n<\/ol>\n

    Supplemental results<\/em><\/strong><\/p>\n

    In addition, the infrastructure acquired by the current project was used to complete the experimental part of one PhD thesis and one Bachelor thesis. Both students were in part coordinated by Dr. Brindusa Petre, the director of the project.<\/p>\n

    PhD thesis: \u00a0Sabina Bancila, \u201cContribu\u0163ii la studiul extrac\u0163iei \u015fi aplica\u0163iilor biomedicale \u015fi analitice ale zeinelor din porumbul autohton\u201d \u2013 examination date 20.12.2016<\/em><\/p>\n

    Bachelor thesis: Cornelia Irimia, ” Proteomica: metode modern de identificare \u015fi caracterizare a proteinelor” \u2013 examination date February – 2017<\/em><\/p>\n

    Participation at scientific events where the financial support of the project was acknowledged.<\/em><\/strong><\/p>\n