The emphasis of this project was the design and synthesize of novel family of antimicrobial agents with hybrid azaheterocycle skeleton as an alternative approach to the next generation of antimicrobial drugs, using eco-friendly methods.

The novel antimicrobial hybrid azaheterocycle will contain in the same molecule a six member ring Π–deficient rationally substituted (di-)azine and/or a five member ring Π–reach rationally substituted (di-, or oxa-)azole and/or triazole. These two main units will be connected via properly chosen linker/spacers, and anchored with different substituents. The reaction setup procedures involve several types of typical organic chemistry reactions: Sharpless click chemistry, N-acylation, N- and O- alkylation, 3+n Huisgen cycloadditions, quaternization of nitrogen heterocycle.

These reactions will be performed by conventional and nonconventional methods, and there will be elaborated new environmentally friendly setup procedures of work, particularly using microwave (MW) and/or ultrasound (US) technology. There will be obtained several new classes of antimicrobial agents with hybrid azaheterocycle skeleton.

The new classes of compounds will be test for their antibacterial and antifungal activity, for toxicity and for leading structures a complete
ADMET (these include Plasma Protein Binding, Caco-2 Permeability, Cytochrome P450 Inhibition and in vitro Microsomal Stability)
will be performed.